RESUMO
Objective: To investigate the relationship of vitamin D deficiency and risk of AMI in a Pakistani population, and to find out any association between vitamin D binding protein [VDBP] genotypes and risk of AMI in this population
Methods: In a comparative cross-sectional study, 246 patients [age: 20-70 years; 171 males and 75 females] with first AMI were enrolled with informed consent. Similarly, 345 healthy adults [230 males and 115 females] were enrolled as controls. Their fasting serum samples were analyzed for 25 [OH] vitamin D, lipids and other biomarkers using kit methods, while DNA was analyzed for VDBP genotypes using PCR-RFLP based methods. Chi-squared test and logistic regression were used for association of vitamin D deficiency and VDBP genotypes with AMI
Results: Mean serum concentration of 25[OH] vitamin D was significantly lower in AMI patients compared to healthy subjects [p=0.015] and percent vitamin D deficiency was higher in AMI patients compared to healthy subjects [p=0.003]. VDBP IF-IF genotype was positively associated with the risk of AMI in subject above 45 years after adjusting for potential confounders [OR = 9.86; 95% CI=1.16 to 83.43]
Conclusion: Vitamin D deficiency and VDBP IF-IF genotype are associated with AMI in Pakistani adults